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CRIMIC CR70179

Gene wal

CG8996

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:11688569..11690863	48C1-48C2	FBti0216964	93370

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	OK	GCGATCTGGTAGAAATCAAACCG	CACAAAGGTGTCGTCCGACTTA

Images

Construct Design Sequences

sgRNA sequence	TGATCAGTGGGAGTCGCTCTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTCCAGTTTTCCAATTTTCATACTAGATTAAAACTAACTAAAAATTTAGAAAAAAATGTTTTGATTTTGGATTGGTGAACCCTTACTTAGATACAATATATAGTAACAATATTTCAAAAATGACGAGCAAAGTGATTTCAATAGATGACCATTTTAAAATACCAAGACATTCAAATTATAAAGCATCGAACCACCCAAGA	GCGACTCCCACTGATCATAAGTATTTGCTGCGATAGGTAATCTTACATAATCACTCTCTTGTTTTCCAGGCTTCCGAGGCTCTTTCCAAGGTGGATGGCGTGACCAAGGTTCTGGTGGCCGAGAATGCCGCCTTCAACGGATTTACCGCGGAGTCGCTGACCCCATTGGTCCTGGCCGCCCAGTCTCAGTTCAAGTTCAC

 

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