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CRIMIC CR70180

Gene AsnS

CG33486

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:21173118..21174973	78C1-78C2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCTAATCTCAGGGAGCTGTTACCT	TCCACATTAGTTGCCAAGTGGAGC

Images

Construct Design Sequences

sgRNA sequence	AAAGATAAGCGAGAAATGTGCGG
sgRNA2 sequence	CAGACTTGCCAATTTACTTAAGG

 

left_200bp	right_200bp
GTGAAGGAACTAGTTGCACGTGGAAATAAGCTCTGTTGACGAGAGTAAGTATAAAAATGGAATTAAATCTAGAGAGCTTTGGCCATAAATAAGAGTGCACGCCATTGAAAAGCTTCATTCAGTTTTCGGACTTTGCAAAGTGCGGTCCGCTTCGTTAAAAAAAAATACAGTTGAAATTACTGCAAAGATAAGCGAGAAAT	GTAAATTGGCAAGTCTGAGGAATTCCAACAATTATTTGCTGAGACATGCACAATAAATTTCTTTAATTGGTAGTCAATTCACTAATATACTATGCGATTCCGTACATCTGTGGGTATTCGGTTACAGATATAGATATATGTATATATATATATACATATATATATATATGTATATATGTATATATGTATGTATATATATA

 

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