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CRIMIC CR70187

Gene Acsf3

CG18155

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:7329046..7331255	7B1	FBti0217656	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTTGCAGGATGGCTTCATCGAGA	CATCGATGTCGAGGATACACTCTC

Images

Construct Design Sequences

sgRNA sequence	CCGGACGTTCAATTTATTTACGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGTTTCGATGAAGAGGAGCGTAGTAATGTGGTGGTGCCCACGTTCAAAAAGGCTATGCTCTATCCGGATGAGATCGCCGTGAAAGACATCAATGGGGAGTACACTTATTTTCAATTGTATATGGCCGCCAAACGTCTGGGCATTCAGATCTCAAATATATGTGGTAAGTGACAAACCATTTTTCCGGACGTTCAATTTAT	TTACGGTTCAATTTCAAAAAGTTTGCAAATTCTTCTCTTTTTGCAGGCGGCGCCGCGCTTTCGAATGTAACATACTTGTGCTCGAACAACGCCCTGTGGATAGCTATACAATGGAGCTGTTGGATTTCCGGACAAGTGGCAGTTCCACTGGAATCCGGCCAGGCGATAGATCAATTGCAACGGCAGGCATCGAACTGCAA

 

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