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CRIMIC CR70188

Gene CG33253

CG33253

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:19183421..19187278	18A5-18A6	FBti0217657	93795

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCAGCAGTTGTAAAGATACATCC	TCACAGTAACCGAGTCCTTGGAGA

Images

Construct Design Sequences

sgRNA sequence	TGGTTATGATGATGATTAGCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTCAGAAAGTTATAATATACTCGTATGTTTTTAAGCTAAAGTTAAATGCAAAAATGGTGTCCATTTTCATAACCAATGATATTAATTGCATATCTATTGCTTAAGCTTTTTTAATAAATATTAAATTAAATCCAAAATATTACAAAATGTTGTGAGAATTGATTGTCCTAAGCAAAAGTTTAAAAACAATCATACCAGCT	AATCATCATCATAACCAATCATACCACTTTACATACTGCATACGATGATCTTCAAATCTTGCAGCTGAAAACGATGACATGGGCTGCGTGGAGATCCTGGCCACGGTGGTGTCCGTGCTGATCATGGTGCTAACCTTTCCCATCTCGGTGTTCATCTGCTTCAAGGTGGTCTCCGAGTACGAGCGAGCGGTGATCTTCCG

 

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