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CRIMIC CR70192

Gene pAbp

CG5119

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:18140078..18147191	55B8	FBti0216966	93372

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGACAAGTTCTCGTCTGCTGGTC	GCAGTTTGGGCAGTATGGAACCAT

Images

Construct Design Sequences

sgRNA sequence	GGCTAAACGCAAGTCCAAACTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TATGCCTACGTCAACTTCCAGCAGCCAGCCGATGGTGAGTAATCCCCGCAAAAGTAGTGTAATTTTTTTAGTCTTACTGCCCGCAAAAGTAGCCCCCTTCCGCGCGAAACAAAGAAAACACGTGTGCAGTTTTGCACTCGCTTACTCACACACGCACGCACACTAGCGCATGTCATACAGTGGGGCTAAACGCAAGTCCA	AACTGGACTTTATTGAAAGATGTCTTTCATAGGAGACCAGATAAACGGCTTCCAAGTGTTTCATAACTTGTTCCACAGTGCACTGCTTACGTTAATCTCGTCGCGCACACACACACGCACTCAATTCCCGCCAGTGTGTACACACACACACATGATTATATCAGTTCCAGTTATTCTGGATGTATACGATTTGTGCGGGG

 

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