CR70194

CRIMIC CR70194

Gene Pex16

CG3947

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:20776766..20778264	77E6	FBti0220427	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGATGCCGAGACTAAACAAGCAA	ATCACTGGCAACAAGAAGAGCCAC

Images

Construct Design Sequences

sgRNA sequence	ATAAAAATAATGAGGTGTTTCGG
sgRNA2 sequence	TAGAGCGTTAGTATTCCCCTAGG

left_200bp	right_200bp
CTCGTGACACTGGTATTTGGTATTTATTTTTGTACCAATTTTATTAATCCAAAGCGTTTAATCAACGCTCTCCGTGCTTGCATTCATATATGTATTCATTTTGCGTCACCTTCTATTTTGTGTTGCATGAAAGCACTAACGCCTTGTTCAAAACGCCAGAACACCACAACTGCACCGATTTGATTACCTGTAGACCGAAA	CCTAGGCTAAGTTTTAAAGTTACAGGTGTGGACGCATTCGGAGTGTTGAGGGTGGGTTGAGTTTTGGTCCTTGAAAAGTTGTTTGCACAATAAAACGATGGAGTAATTTTCGATATGTTTATAGCCAACTTTCATATTCCTTGAATTGACCCACCCACATGGCATTGCCGCTCTTGAGGTGAAAAATGATTATACATCCC