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CRIMIC CR70194

Gene Pex16

CG3947

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:20776766..20778264	77E6	FBti0220427

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGATGCCGAGACTAAACAAGCAA	ATCACTGGCAACAAGAAGAGCCAC

Images

Construct Design Sequences

sgRNA sequence	ATAAAAATAATGAGGTGTTTCGG
sgRNA2 sequence	TAGAGCGTTAGTATTCCCCTAGG

 

left_200bp	right_200bp
CTCGTGACACTGGTATTTGGTATTTATTTTTGTACCAATTTTATTAATCCAAAGCGTTTAATCAACGCTCTCCGTGCTTGCATTCATATATGTATTCATTTTGCGTCACCTTCTATTTTGTGTTGCATGAAAGCACTAACGCCTTGTTCAAAACGCCAGAACACCACAACTGCACCGATTTGATTACCTGTAGACCGAAA	CCTAGGCTAAGTTTTAAAGTTACAGGTGTGGACGCATTCGGAGTGTTGAGGGTGGGTTGAGTTTTGGTCCTTGAAAAGTTGTTTGCACAATAAAACGATGGAGTAATTTTCGATATGTTTATAGCCAACTTTCATATTCCTTGAATTGACCCACCCACATGGCATTGCCGCTCTTGAGGTGAAAAATGATTATACATCCC

 

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