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CRIMIC CR70196

Gene rin

CG9412

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:13647004..13654527	87F6-87F7	FBti0216967	93373

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCGTCGTACAACAACAGCAACGG	TTACGCAGTTCGCCAGGCACTTTT

Images

Construct Design Sequences

sgRNA sequence	CTTTCTTATCAACTTAAGGTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGGTCATTCCCCTCTATTCGGCTTCAGGGTAAAAAGACTAAAAGATGTAGTGCAAACTTTTGGATCTCATGTAGAAATGAAAACAGTGGTTTCTAAGAATTACTTTTTTAGTCCCCTTATAATTTATTATGATTGGCTTACGTTCAGATTTTTAGAAAGGTAATGATTCATTTTGTTGGTTTACTTTCTTATCAACTTAA	GGTTGGCTTATATGATAAGCAAAATTGGGTACCGCTTTTTAGAAACTCTTCCTAATAACTTGCTGTTTATACTTTACAGACTTTGAGCAGCGTCGCTCGAGCAACACGCAGCAGTTCGGTGATAATCAGCAGCTGTTTTTGGGCAATATACCGCACCACGCCAGCGAGGATGATCTGCGCGAGATCTTCTCACGCTTCGG

 

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