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CRIMIC CR70199

Gene Dbct

CG5599

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:15086125..15088629	13A10	FBti0216969

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCTCGATCGCATGAGGCTAAA	CAGCGTCTCAACTGGATGGAAT

Images

Construct Design Sequences

sgRNA sequence	TATCTCTATACTCTTCCAATCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTAAATGGCAACACACCTCCTCCGAAACGGCGCTACATGTTGGCTGCTCAGGAACTGCATTTCCCAACGTGCCGCGGTGAGTAGCCCCCAAAAACCACCCGCCAATCGGTGGTCAAAAGAAAAACAACACCGCTGTTATGTAATCTCACAAACACCTTTCGCGCGCGAAAGCGAGAAGAAGAATATCTCTATACTCTTCC	AATCGGCGGAATTTGTTTTTCTTACACAGAGGAAAAAAGTGAGATCAAAGATCATCAAGATTCGGAATATATCCAAAAATTAGTTTATTCTCAAATAATTATTGATTACTTATATATATATATTGACATATATGTATGTCAATTTTTAACAATAAAATAAAATAATATATTAGAAGTCATTTTGTATATTGTATAATTAC

 

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