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CRIMIC CR70202

Gene GM130

CG11061

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:22124567..22127502	58B10	FBti0217658	93798

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTTGGCTCTAAACGCGTACGGTC	TTTCAGCGATGATCACCTGGATGG

Images

Construct Design Sequences

sgRNA sequence	TAAACAAAATCAATATTAGTCGG
sgRNA2 sequence

 

left_200bp	right_200bp
AATTTACATTTGTTGGCTCGCCTTCCGAACCGAGCTTACTCCAGGTGTGGCAAAGGTACCGCTGCTGACCCCGAAGGACATGCCCGATGACACGCAGGATGCCAAGGCGCAGAAGTTGGCCGCGGCAAGGAAAAAGGTGCGACAACCAGTGCCACACACAATTGTTTTGCTCTTGTGACGTGGACACATATTGGCCGACT	AATATTGATTTTGTTTAACACGCTTCTGTGGACACAGCTGAAGGAGTACCAGCAGCGCGCCAGCAACAACAATGGGCAGCCGGGAGCAGAGGAGCCGGTGGCTGCCACCACGCAATCGCACTCGTCCACTGTGAGCATATCCAGCAATCTGTCGGAACGGTCAGACAGCGAGATAAATGTGAACGGTGGAGGAGCAGGTG

 

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