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CRIMIC CR70205

Gene nocte

CG17255

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:10461865..10474675	9C5-9D1	FBti0216971	93377

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTTATCTCAAGGGTAACCAGG	TGAAGCACCTGGTTCTGCAGAT

Images

Construct Design Sequences

sgRNA sequence	TTAAGACGCTCAATAAATGTCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAGGACTAGATACACTACCACCCCGACCAGAAATCAAAATAAATTCAAGATCACTATCAAGCTAAAGCAAGATTCGAAGCCAGTAAATTTCCTGAAGAAGTAATGTATCTGATGAAATTATCCATGAGTGTCGTCATAATTTTTGAAACGAATTCGTGTTATTGAATTTGGCTTTATGTACGTTTAAGACGCTCAATAAA	TGTCGGATACATTAGTATCAATTTGTTTTCATACTTTAAAATCATTCAAACGTACTAAACCGGATAAAATTAGAATTAGCCATTGATTTATTCTTTTTATATGCTCCTAATCACATGCTTTCATTCGCAGATGGGTCGTCAGCTGAAGAGTCCGCTGAGCGCTGACATGAGCCTCGGTCTGGCCAAGCAGGTGCAGTCGC

 

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