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CRIMIC CR70207

Gene Pex1

CG6760

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:14071892..14076123	70D1-70D2	FBti0216972	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATGTGCATCGGTTGCCAATGT	TGTCAGCGCAACTTGCATCGACTT

Images

Construct Design Sequences

sgRNA sequence	ATAGACAAGTCCGTTTATATCGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACTTACCCTTTCTTGCCGTAGGTCTGCACGAGAATGATCTGGTCAAGTGTGCGCTCATCGCTGACGTTCTCAACCTGCGCAGCGTCCACGTTACCCCCGTCTCGTCCAAGGACTGGGAGATCATTGTGAGTGACGGTTTCGCCTGCTTGGCGGCGTGGCCAGCGATCAGCGGTCAGCTTAAAAATAGACAAGTCCGTTTA	TATCGGATTAGCATTTGGCGCGGGCGTGCGCTTGGTCTTTATTTATACTGCCATTGCTTGGCCGGCATTGCCATTGACCCGCTGAATTGATTGGATTAAATTAACCAATTAATTAACCTATAATCAGATTTCCCCTTTCCGTAAGGCCCTTAATTCATATACTTGCTTTTATTAATATGAAAACACACGTTTTTGCAGGA

 

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