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CRIMIC CR70210

Gene Roe1

CG6155

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:13554077..13555606	50B1	FBti0217659	93799

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGAGCTTCTGCAAGGATCTGCT	ATGCAACGCTCGTGCAGTTTGTAG

Images

Construct Design Sequences

sgRNA sequence	TTGTACATATAGATTTGTTGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTTCTAGGTTTAAGGTATCTTAAGGATTCGGGCTCGCAATGAAGTTACATATGTTGAGATTAGAATTTCGTAATTGTTTGTTGCCAATACTTGAAAATCTTCATAGCATTTTAAGGTGTATTTTACCGATCTTTCCATTTCTATCTGAGAGATTACAAACCCGATTGCATTACATACATCTAATTGTACATATAGATTTG	TTGGGGTATTTTAAATTGAGATATTTGGATAACTTGTTAGCCATTTTAGACTTAATAGATAGTTTAATCGTTTTGCATTACCATTTTTAAAACTACCCTTACTAATGATCTTAATTCTTGCAGCTCAGCGGCAATGCTGATTTGAAAAACCTGTACGAGGGTCTCACCATGACGCGCGCCTCGCTGCTGCAGGTGTTCAA

 

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