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CRIMIC CR70212

Gene Vha68-2

CG3762

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:12974322..12978607	34A3	FBti0217660	93800

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAACTACAAGGTCGACGATGTCGT	AGGTGGAATCAGCCATCATGGACA

Images

Construct Design Sequences

sgRNA sequence	GAGTGTATGGAGGCTACACGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCCAGTGCGTCAGCCACGTCCCGTGACCGAGAAGCTGCCCGCCAACCACCCCCTGCTCACCGGACAGCGTGTGCTCGACTCGCTCTTCCCCTGTGTCCAGGGCGGTACCACCGCCATTCCCGGAGCTTTCGGTTGCGGCAAGACTGTGATCTCGCAGGTGAGAGTCCCACAAATTGAGAATTTAAGGAGCGATGCCTCGT	GTAGCCTCCATACACTCAAGTTTCATAAAAACACAATCCCTAATAAATCATTTACTTGCTTGCAGGCCCTGTCCAAATACTCCAACTCCGATGTCATCATCTACGTCGGTTGCGGTGAGCGTGGTAACGAGATGTCTGAGGTACTGCGTGACTTCCCCGAGCTGTCCGTGGAGATCGACGGTGTGACCGAGTCCATCATG

 

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