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CRIMIC CR70213

Gene Sp1

CG1343

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	X:9729648..9756369	9A1	FBti0217694

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCCGTGGAAGAAGAGTCCCAA	TACGGATGACGCGAATAGACAC

Images

Construct Design Sequences

sgRNA sequence	AAAAGTGGTCAGGTGGTGGATGG
sgRNA2 sequence	TTTGATTTGAAGCTATCTTGCGG

 

left_200bp	right_200bp
GAGCGGATCAAGCGGAGGACAGTCCTCCAGGAGCCTCTCATCCAGCGCCAGCACCATGGTTAACATCACAGCTAGGTAAGCAGACAGGCGGCGCACAGCGATCCAGCAGGGATTGCCCAAGTGAACGGTGGTTGGATGGGGGATTCTGGTGGTTTGGGATGTTAAGAGTTTAATATATTCCATTTACTAGACATCCATCC	TTGCGGTTGTACATATAGCCAGCAGATGTCTGTAAATAGATTTGAGAAAGAAACGCCCGCTCTATGTGTCTATTGCGCTAATCCCAGCACTAAACTAAAACTGAAAGTAAAATATTACAAAATATAAATATAAATATAAATATATTGCTTATAGCCTATTTGTAGACTAAGCCATTTTCTAGACTATCTAGTTATCAACA

 

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