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CRIMIC CR70219

Gene LpR1

CG31094

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:25741348..25763105	96F1-96F2	FBti0217663	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
	OK	GTCCTGCTATCGGTGGTAAGTTGA	TTAAGGATGCGCTCCAAGGTGA

Images

Construct Design Sequences

sgRNA sequence	TTCCCTTCCATAATATTAATCGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACCCCATCTACCGCAAGACCACCACCACAGAGGATCACTTCAGTTTGCGCAAAAATCTGCCGGCGCGGATCTACGACCACACCAGTGTCATGGATGAGGAGGTGGGTCTCCAATTATTAATTACCATAAAAATCCAAGTATAATGATAAGCATTGGGTTTAACACACCCGCAAATGATTTAATTTCCCTTCCATAATATT	AATCGGTATACTTATTATAATTTATCTTAATTTATCTTGCAGTACTCACCCGTCATAGGCATATCCTCGTATTAGATTGATTCCTCTGGAATCGTGTGAAATGTGGATTGGATGATTGTTGCGCTTGGTCGCTGGGAAAATTAGATGACCAATGCTAACTCGAGACCCGCGGCAGAAACACACACGCCTGAAGACTGAGC

 

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