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CRIMIC CR70220

Gene LpR2

CG31092

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:25698727..25740465	96E10-96F1	FBti0217664	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTGTGAACGTCGTAGCCAAGT	TTGTTGTCGGTTTCCGCTCGGATT

Images

Construct Design Sequences

sgRNA sequence	AAGAGCACGCATTTCAAAGCGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAAAATGCCGCGAGTAAAACGGAACCGCAGTACCAATTTAGATATTAAAAACGGTAAGTTGTGGTCACAAAATCCATATTAGAACATTATTAGCGTATTGTTTTACATAATTATTGTCTCGTGTGCGCAATTTAATTATCATTTTATTGGCCTTTAATTTAATTTAATTTACCATCGATTTGAGAATGGCAGCGCCCGCT	TTGAAATGCGTGCTCTTTGAATTTACTGCCCGTGGGTCTCGCTAATTGCAGAGCTGCAAATGCAATCAAAATATGCATATCAATTGGCCAATTAACTAAAAGGCCAATTAAATGGCGTCAGTGGAGCGTCAAAATATCCCACAGCCCGACAGCTGCCCAACTAAAATATAAAACATAAAGCGACTCACTTTGGCAGCACA

 

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