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CRIMIC CR70222

Gene Vps29

CG4764

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	2L:1150766..1151764	21F1	FBti0220430

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTAATGGAAGAGCCATTAGGTGGC	TCCAACTGTCGTCAGCAGCAATCA

Images

Construct Design Sequences

sgRNA sequence	CGCCCAGCGAATTAAACGATAGG
sgRNA2 sequence	AATGGCTGGTGGCTAATACTAGG

 

left_200bp	right_200bp
CTTGTTTAAAACAAATACCTTCCTTGCTTGCTGCTTGATCTGCTTCCATTTTCGCCGGCAATGTTATCGCTAGAGATGCGAACCAATCGATATTTGCAGTAGGGTAGCGCAATAAACTTACGTTTTATTTATTGAAAACAAATCGGTGTCACCACCAGCCGCCTTCGATAGAGCTGTTATCGTTATCGCAGCCACCTATC	ATTAGCCACCAGCCATTCCCCTCCAATCCAACCCTTGACCCCTGCATTCTGAGCTCGGCTTCATTAAAGAAATTGTAAATGTACGTAGCAATAAATCAAGTTCTCATTTTTTTATCTGATAAAAAATGCTAGCACCTTGTTTAAATAGAGAATAAAAGTACGAACGGACGAAAGTGAAGTTTTAGATTTAATGCAATAAT

 

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