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CRIMIC CR70223

Gene AP-2sigma

CG6056

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:21269209..21270075	93D2-93D2	FBti0220431

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGTGAGACGATCGCTTCTGCACT	GACTGGCTGCTGTTCAGAGTGTTT

Images

Construct Design Sequences

sgRNA sequence	TGAGCAAATTGTGTATCACTGGG
sgRNA2 sequence	ATAGGCGGGTGTAGCGCCTGGGG

 

left_200bp	right_200bp
GGGGCAACAGGAGATTCAAGCGAGAGGCCAACATTTTTGGTAAATGACTAGTTTGGAGTGGTGGCCAAAACAAATATGAAAGTGAAACTTCGTAATACAGCGCGCGGGTCTGCCAACTCGTTTGCTTTCTGCGCGAACTGTTTGCAACACTGACGAGGTAACAGCTGTTCCGGAACAGTAGTGGTAGCCCTGGTCCCAGT	GCGCTACACCCGCCTATCGAATCTTCTTTCATATACTATTACGCGTATTATTATTATTATCTTTAATGTGTTCTCTATGCAAACACTCAAATGTTAAAGGCCGTGTTTAGAAACAAATGATGTACCATAAGAGATTGTATGCAAAAGTTAAGTATTTTTGTTTTTATATGGGGTGTGTTAGGGGTCATTTGGCGATAATC

 

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