| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_pM37_p2 | 3R:6209373..6213493 | 83E1 | FBti0217665 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | CCAGCAGCAACAACAGTTTCCACA | ATTGTGACAGCTTACCGTTCGG |
 |
| sgRNA sequence | AATATGAAAGAGGCATCACTAGG |
| sgRNA2 sequence |
| Amplicon PCR 5' Left | Amplicon PCR 5' Right | Amplicon PCR 3' Left | Amplicon PCR 3' Right |
|---|---|---|---|
| Amplicon 5' | Amplicon 3' |
|---|---|