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CRIMIC CR70233

Gene cac

CG43368

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:11931280..11985087		FBti0217668

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCTACAAAGAGTTCTGGTACCCA	AGAGTACGCATTGGGTGATGATGC

Images

Construct Design Sequences

sgRNA sequence	GGGTATAAGTATATAACTGGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAACCAAACAATCAATAATCTAATCCATATATACTCTGCAGTGCGTAATGCATATTATATTCTCTGCATTTTGCCACACACTTAACCCGATTTTTACTACCATAGCGATAAAGTTGAATTCCCCCCCCCCCCCTGTCAAAATAAACCAAACTAACCAAATACATAGCTATTTCAACTGCAGTTAGCCAAATTAACCTCCA	GTTATATACTTATACCCGCCAGCGGACTCGTGAATTGTTGTTGAGTCTCGGCCTCGTTCTCGATATCTTGGCTAATATAGTACACTCCCTATACACTATATACACTGATAGAACAGTGCACCCACCTATCAATATCAATGTTCTGTCCCCGGTTAACTATCGCCATCAAAATGTATCATCGCTCCAGACTCAGAGACTCC

 

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