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CRIMIC CR70234

Gene CG5800

CG5800

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	X:17636281..17639003	16B10	FBti0220979	95003

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATCGACCTGTCCCATTGGACTTT	AACCGAAAAGAAGTCTCCTCCCTC

Images

Construct Design Sequences

sgRNA sequence	AACGTATTGAAATTCGCACGCGG
sgRNA2 sequence	CAAACTTCTATTAAGCTACACGG

 

left_200bp	right_200bp
ATTTATATTTCCTGGGAATATTTATTTAATGTTTATCAATCGAGATCCCGAGATCGAATCGAAGTAAGAAAGTAAGAAAATTAATTCTTTCCAAATTTCTAGAAACGGTAATACAAATTAGTGGTCGATACATTCGACTTGAAATGCGAATTAATCGATTGAATAATCGTTTCTTCTCTCATCCCTAATTCCAACCGCGT	AGCTTAATAGAAGTTTGTTTTTATGGATACTTCAATTACCGATGTTATTTTCATGGGGCTCAATTAAAGGAAACTTCACTTTAATTATATAAAGAAGTTTGGAGCTAAAAAGATATATTTGCATCTGAGGATGTTCTGAAATGCTTTTTTGTTATCCCCAGTAAAAAGTCGCTTTTCAAATGCTTCACAAATATTTGGAA

 

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