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CRIMIC CR70237

Gene Fitm

CG10671

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	3L:5144082..5146063	64C7	FBti0220433	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAGGAACACCGACTTGAACTACA	TCAGTTCTGGACCTTCGGAAAGCT

Images

Construct Design Sequences

sgRNA sequence	ACAACTGAGAACAACGCAGTGGG
sgRNA2 sequence	GAGAGCTGAGAGCTGAACCCTGG

 

left_200bp	right_200bp
CTCGAGATAAATCTCAGGCCCGAACAGCTGATCACATGGCTTGCCATTCGCCACGAAATCCGCTCTTTATAAAAATTCCAATCAGAGTTGCCAGTCACGGTGAAGCGTGCTCACCAGCTGCAGCTGTTCTATTTTTTACAGGCGCAACACGTACATTTGGGCCAAAATAAGAATTGAAAAATTACAACTGAGAACAACGC	CCCTGGTCAAGTCAACAGAGGGGAGAGCAGCCATCAAGCATAGAAGGAATTGATAGTTAGGTGACGGATTGGAATTTATACCAAATTTTTGTATATTCAGCAACGATGATATGAAACGATGATGATATTACTAAGCAAGCAGCCGAGGCTGGCACTGGATCGCGCGCTTTGCTCCTCATCCTCCTCCACCGCCGGATTCT

 

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