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CRIMIC CR70241

Gene sp3

CG7956

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:21520928..21528598	93E4	FBti0217669

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCTATCTCATTTGGGAGAACCAG	GATTGCAGTGATCCCTAATCTCC

Images

Construct Design Sequences

sgRNA sequence	ATTACAGGCTTTGTCACTTAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TATAAAAGAATTCCAAGGATGCACGTATCCGGCACGTAAAGTGCAATTGCATATTGCTGTTTTTAGCTGCAGCAGTGGGCTGTGTGGGTGCCTTTCAGATTCGCTGCACAGTAATGAGCGGTTGTAAACCCAAGGGGAAATCATTCCACACGCAACGCCCCAGATGCAAATGTGTAACCTGAAATTACAGGCTTTGTCAC	TTAAGGGCAGTCCTCATTAGTCGGCGGTCAATATTCGTGCTCACCCAACGAGTAATGCTCTAGTTATTTGTATAAGCTTTGCCAGTTGTTTATTCTGAGAGATTGTTTTATTCAGGGGGCGTACGCTAATCAAATCCCAATAAACCCTAACGAGCAAAAACATGGTCATTAGGCAGATGATATACCTACAAATCATTCAT

 

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