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CRIMIC CR70242

Gene lola

CG12052

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:10481894..10543291	7A11-47A13	FBti0217670	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCAATATCTCGCAGGATCAGCTGG	TAACGTTGGTGCTGGTCAATGGCT

Images

Construct Design Sequences

sgRNA sequence	TTTGAAGAACATTCCCGCATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACTGGAGCAAACTAAGCGGGCTCGACTGGCCACCGGCGGAGCGATGGATACGTCTGGCGATGTGTCCGGTTCGCGCGAGGGCTCCTCGAGTCCGTCGCGTCGTCGCCGAAAAGTCCGACGTCGCAGCATGGAGAATGGTGAGTTGTCAAGCGATTAGGATTATCTTACATATTTGCCGAATATAAAAAAAAGTTCCAATG	CGGGAATGTTCTTCAAACGAAATTAGCGTTAATCAAAAATCTTCAATTTGGCACAATTAATAAATTGAAATTGCTTTGCTAATAATATATTTATGCAACACAACAAAATTTCCTCCTAAGTACAAACTTATTTATTAAAATTGTTCTTATTTTTTTGCAGATGCCCACGACAACTCAAACTCGTCCGTGTTACAAGCCGC

 

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