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CRIMIC CR70243

Gene sli

CG43758

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:15869495..15922118	52C9	FBti0217671	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGAAACACGGCAGAGGTGTTCAC	CGCACGATGTCCAAGTTCTTGATG

Images

Construct Design Sequences

sgRNA sequence	AGTGGCTGCAACGTGAGTTGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGTTAATCATATGGTGGCACATGCTAAATATTAATACTACACCAGCAAGCTTTAATTATTGACACATATACGCACACGCATATTCATGACATTCGCTTTTCACGCTACTCATCCATCAAAGGAAAATATCGTGGGGTTTTTGATGGGGGGGGGCGGAGACGACACTGTGCACTTTGTTAGTTCTAATTATCCGACCGCAA	CTCACGTTGCAGCCACTGCCTGAGCTCGCCGACTAATTGCGTTCTGTTGACTCTTTCCTTCCACCCACAGCGGCGTCCACCTGTCGCAAGGAGCAGGTGCGCGAGTACTACACGGAGAACGACTGTCGCTCGAGGCAGCCGTTGAAGTACGCCAAGTGCGTGGGCGGCTGCGGCAACCAGTGCTGCGCGGCCAAAATTGT

 

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