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CRIMIC CR70244

Gene Dl

CG3619

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:19302731..19326217	92A2	FBti0217672

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCTCCTTGCGTTGTTTCTCTTCTG	AAACGCAGCATGGGACAAGAAAGG

Images

Construct Design Sequences

sgRNA sequence	TGCGGAGAGCGGTAGAGGATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAGTTTCAAAGATAAGAAAATATTAAATTATAATAAATCTTGACCATACATAGTATGTGTACTTAGCATACATTTCATAGTTCAGAACTATTTAGCTACGAAGAAATTACAACTTAGATCCACAATATTAAGCTGATAGGGAGCTCTTTATTGATAATAGTACCCATTTACTCACCGTGCACCCTGCATGTGCTCCAATC	CTCTACCGCTCTCCGCACTTCTTGATTTTCTCCCAACTTTGGCACGTTGCGTGCTTAAACTTCACTCTTCGACCAGCTTCTTTTTGCAGTCTGCTATTTAGTCGCTAATTTTAACAACGTCCCAAAAAAGTGTCTCGACGCCAACCTCCTGGGGTCTTGGGACTGCTGAAAATGCTCCCCGTATGTAGGCAGAAACATCT

 

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