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CRIMIC CR70245

Gene CG5254

CG5254

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	X:750347..758719	1C4	FBti0220435	95006

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GTTGAGCTTTCTGCTGGCTGAGAT	AACAGAATCCCTAGCAAGCTGCTG

Images

Construct Design Sequences

sgRNA sequence	TTGACCCGTAGTGTTCTAGTTGG
sgRNA2 sequence	AAGAAGGCGCTTGCCTCTTGAGG

 

left_200bp	right_200bp
TACTACACCGACAATATACTGCTTACAGATTTTATAATCAACGAGAAAGAAAGAACTTTTTCTTAACATCGAATAGATATACATGCTTTGTGTTCAGTGTGGCTTTTTCTTCTGGAACACAAGCAGCTGCCTCAACCTCGAGCTCAGTGCGAATTGCGTTCTCGAAGCGAGCGGTGTCGGTTGTTGACCCGTAGTGTTCT	GAGGCAAGCGCCTTCTTTGTTTTTTTGTATAATAGTAAGTCGATTAGTCGCGATCAATGCCATCAAGGGGATGCTACTCTTCCGATTGTGATTCGTCAATAAATGTATTTTTACTTTGTGTTGAAAATAAATAATCAATGATATTTTTAGAAAATTTTTAAATTTAAATATGTAAATTTAATATGTAAATTTAATATGTA

 

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