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CRIMIC CR70246

Gene Csp

CG6395

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:22267382..22273745	79D3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTACTTGGCTTAGCCAAGTCC	GGAGAGAGAAACGTTGGAAGCA

Images

Construct Design Sequences

sgRNA sequence	ATGGTTAGGCGTTTATTGTTCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAATCCGGTGCAAAGCTAAAACATTAACTTTAACGCATGCTTCCCATCCACCCACACACTGTACTATTACGAACCTAACAAACCAACTTTGATGATTTTTGTGGGGAGATCTGTATCATTTTTCCTTCTGAGAAGCTAATGTTTAGTCAAAAACGAGCCCCTACATCTGACATCAACGCTTACTTAACAGCTGCCCGAAC	AATAAACGCCTAACCATTTTTTAGTATAATTTTCCTGGGTTATCAGCTATCTTGGCACACCATCTCCCTCGATTGGTTTTCCCTTACTCACACTACTCCGTACAAACACTACTCTGATTGCACTGCTTTGTAATTAGTTTATTTTGGGTAAGTATGAAGTTTGGTTTTGGTTTCCGATTATCAATTCTCCAGACACTGAA

 

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