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CRIMIC CR70252

Gene mtm

CG9115

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	2L:6034731..6037015	26B3	FBti0220438

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCAGCTGCGTTTCAGTTCTTGTGG	GGAACTCTAAGAGTTCCGAACGCA

Images

Construct Design Sequences

sgRNA sequence	AATTGCATTTAGCTAACAATTGG
sgRNA2 sequence	CTACATATGCGTACAAGTAGGGG

 

left_200bp	right_200bp
TGTTCTAGCTTTCCTTGATTACCAGTTAAATGTCTGAAATAAGTTGATTAATTCCAATACATAAAAATCAAATTTTCCGCCACTACATAATGTCCCTTTGGTACTTTTGTCGGAGGAATGGGAAATTATTCCAGATGATTTTATATTGCGTGAGTTGGCAGCACTGCTGCATGAGTTTTGGTGAATTGCATTTAGCTAAC	CTTGTACGCATATGTAGTGCACGTATTGTGAACTACGTTCTAATTCCAAATAAAAGTTTTTCTTAAATGGAAAACTAGTCGATTCAACATTGTATCATTGAACCACATTATCGAATCTAATTAAAGTGTTAAATGTCTGAGAATGTTTTAATTATTTCATGAATAACATCATATAGTTCAAATCGTATAGCGTTTTCGCA

 

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