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CRIMIC CR70256

Gene Pis

CG9245

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:15701451..15703770	13E8	FBti0217674	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGCAAAGCTAGAAGATCGCAA	TACGGAGGAGACAGGTGTTTGCAA

Images

Construct Design Sequences

sgRNA sequence	CATACGTATAGAATCATTGGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GATCGGTGAGTGGCTGTTTGTAGGTTTTATTCCGTGCTCCAGCTAGGTAAACAAATCGCATGTTCGATTTATGCGGCGCAATAATAGCGATTCTAGTGTTCCCTAATTGGATTAATAGGGGCTGTTTGGCAGCTAGTGCAATGCGAAGCGCAAGTTCAGCGATAAGAGCCGCTTCGTCCAACCCAATTGAGTCACCTCCA	ATGATTCTATACGTATGATGCACGCATATGTGCTGATTTATATTGATCTTTTCCAGGCTACGCCCGCATCGTTCTGGCCCTGATCGCCTTCTGGTTCATGTCCACCAACTATGTGATCTCCGGCTGGTGTTATGTAACCTCCGCCCTCCTGGACGCCGTCGATGGACACGCAGCCCGAGCCTTTAACCAAAGTAAGCATT

 

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