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CRIMIC CR70261

Gene Tdc2

CG30446

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	2R:6684745..6689974		FBti0217676

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	TCGTTCGCTGAAGCTGTGGTATGT	ATGTGGACTCATCTCATCTGCGG

Images

Construct Design Sequences

sgRNA sequence	ATGTGGACTCATCTCATCTGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGTCCGAAAGCCCTCGATAGCCAACCAAGTGCGAATGGGTCTGTTGACTGTTGTCGACAATTGAATTGTTTTTGGATTGGAGTGGAGTGGCGTTAAGTATGCTGGCTTGTTTACTCAAATTATGCCCTCGTGTATTTGCTTGCGATTAGTTGTTAAAGCAAGGGTTTCGGTGCTCCGTGTGTGCCGAATCTATCCCGCAG	ATGAGATGAGTCCACATCGAAATATGACAACGCCAAACAAACGTTTATTGCTAATTGTGACCACCCAAAAGAATATTATTTGCCCAATTCCACTCTGAGTTTGCCTGTAGAATGACTTGTATGATTTCAGGTTCGTGCTGCGATCCTATGGGATTTCCGGGCTGCAGCATTATATACGTCACCATATCAAGCTGGCCAAG

 

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