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CRIMIC CR70262

Gene t-Grip84

CG7716

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:7588608..7590998	66A6	FBti0220439	95009

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTCGAAGTTCCCATCGCGTAGAA	GTCTGCAACTGAGGCAAGTGCATT

Images

Construct Design Sequences

sgRNA sequence	CCTTAGTTATTATAGTCATTCGG
sgRNA2 sequence	AGTAAAGATCACGGGTGATTTGG

 

left_200bp	right_200bp
GGATATCACCGAAAGTAACCACCTTTCACAAATGGCTAGAATTAAGTTTGATAATAAGCAGAAATGGAAATCAGAACTAGCAGAAGTTAGGTGTACTCAGCACTAGCACCTATTGTTATAACAACCAAAAACTCTACTAACTCTACATATATTTAATTATTTAATTTCAATCTATCCAACATACCTTAGTTATTATAGTC	CACCCGTGATCTTTACTCTCCATGACCCATGGAACATTTAGTGCTGTGTTTACTTAACTATATTATAAGAGCGCTCTTGCAAAATGAAGTTAACTCTTTCATTTACTTTATTAATCCTTTGAGATTCAAAAAGCTATCAATCCATTATCCACAAGTGTGAGCTTGTCTTATGCGTATGACACCTGTGGCGCTCTTCCACT

 

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