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CRIMIC CR70263

Gene Gdap1

CG4623

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:5141953..5144317	64C7	FBti0217677

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATCCGTACAACTTCCATGCGCAA	CCTTGGGATTGAGGTTTAGGAACC

Images

Construct Design Sequences

sgRNA sequence	ATAAACAAACTTATGTGTGGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTAAATCTCTCGGCCACTCGGATGCAATGGAGCATGACCTAGCGGGTGGCGGGCCGGAAATGTAGTAGGGCTAGCGCTATTGGAGTTCTCTAGTTTTCCATTCTGTACAGATATGACAGCATATTTACCTGCTGGCTGCTCCAGGTGCTAATGAAGTGGATTGAAGCGGAGTGAAAAAACAAAATAAACAAACTTATGTG	TGGAGGAATCTGCTCCAAAAGAACGTAATATAATAACTAAACCGAAATGATTTAATTTAATGCCAGATCGTGTCTAAAAATATCTGTTTTGGTTAATGAAGCGAGTAATTAGTAGCGGAATATTGCATCTTTGGGTTCGTCTTTAGATACATTTAAGCAAATGCACATATATACTTAATATCTGAGTTATAAATCTGCTA

 

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