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CRIMIC CR70264

Gene mib1

CG5841

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	3L:16006552..16011812	72C2	FBti0220440	95010

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGGTAAGCTTAGACACCCATCCC	CAACGACATCCTTTCAACCTGGTC

Images

Construct Design Sequences

sgRNA sequence	GAGTCGCTGCTAAACACTCACGG
sgRNA2 sequence	CGAAGTATTAGGATATTTAAAGG

 

left_200bp	right_200bp
TATATTGGTAACATTCAATCGCTACATTTTTGCGGCAACTCTTCGCACTTTTTCCACTGTGCGGCATAGAGTGACCAGAGTTCAGCAACTGCACTTGAAATTTCCTGCGTAACGTCACACTGTTAATCTATCGCACCGCATGAGATCTATCGCTTGCGCGACTACGGTCACATTCTTCGGTGGTTGTAAAAATTCCGTGA	TAAAGGACTCACATTCTTATACACACATATATACACACACAATATCATATATATAACTATATATCGGATTGGTCTTATAAAGCAAAAATATATTTTTAACAAATATTCAAACAAAAACATTAAGCAGAAATACTTTTGCTTGGCTCCTTGCTAATCAGATCCCTCAGAATGTCCTTCTTGGCCTGGAAATAGTCACGGAA

 

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