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CRIMIC CR70265

Gene Golgin245

CG3493

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:23371360..23377045	59D9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCAGTGTGACCGTTGAATGAACA	GGCAGCTTTTGGATGCGATAAG

Images

Construct Design Sequences

sgRNA sequence	TGTGTCGGTGAACTGGGAAGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CACTCGAAATGTTCGCCAACCTGAAGAACAAGCTGATCGAGGAGGTGAAGGCGTCGCCCTCGAAGTTCCAGCAATTTGCGAATGCTGCACAGGTATTTTTGTGTGCGGGGGCGGGGCGGTGCTTATTGCCGATGAAAAATGCAAATTGAGGAAAACATACGCACACAGACACGGAATTGCATGTGTGTCGGTGAACTGGG	AAGGGGTCGCCTTCGATTGTGCGATTGTGACCCATTTCTGGGTGCACAAGTGAAAAGTGAAGTGACCAGCTGCTCATGAGTCTTCCTTGGACTTTTTTCCCAGGCCGCCGTGAGCTCATCGTCGAGCACAACACCCAATTCGGAAACGAACACCAGCAACAATGAGAACTTCTTCAGCATCACGGAGGAGGGTGAGTACG

 

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