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CRIMIC CR70266

Gene ThrRS

CG5353

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:12103751..12107170	33C4	FBti0217678	93821

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	CGAAAAGTGCTCATTGCCATGTCC	AACTTGAGAAGCTGCAGGGTGCAGTT

Images

Construct Design Sequences

sgRNA sequence	TTCAGGTATTAGAGAGAAGAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGAATATCTCCGGTTTATATTGATTTTTAACCTATTTCCGATGGATTTTCAGGCTGCCAAGATGAAGAAGGAGAAAAAGGAGAAGCCATCCGGCGGTGGAGATACTCGCAAGGGTAAGTAATTCTAACTAAGAATTAAAAACACAAAACGCACTTCCGTCTGTAAGATATGTAGCATATCTTTAAGATTAGCTGCCTTCT	TCTCTCTAATACCTGAAATTCCACTGATCTCTGCTATATTGATTTCAGAACTCAGTCCCTTGCCCAAGTACATCGAGGAGCGCAATGTCTTCTGGGAGAAGTGCAAGGCGGAATACGAGGCCGAACTGGCGGCCAAGAAGCGTGAGCCCATCAAGGTGACACTGCCCGATGGCAAGCAGGTGGACGCCACCTCCTGGGAG

 

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