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CRIMIC CR70267

Gene shop

CG7280

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	X:18846389..18848566	17E1	FBti0220441

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCACATACTCTTTACCTACAACC	CAAGGTCAAGAGTCTGGTGGAGAA

Images

Construct Design Sequences

sgRNA sequence	CGGAGCGGCAAGAAGCAGAGCGG
sgRNA2 sequence	CTGGCAATACTCAAGATAGAAGG

 

left_200bp	right_200bp
TACAGTGTGTTTTGTTGTCGAGGAGCGGCTGGTAAACAGCCGAATTAGCGCTCCCAAGATTGCTTATCAATTAAACCAACGCCGATTGGGGGCCAGAGGCTGCTGGGGCCAATGACGCCACTGGACAAACCACAAGACATATCACTTAGGAGGCCACATAAATCAGCAGCGAAACAGATTCACCGGAGCGGCAAGAAGCA	AGAAGGAGACATAGATAGAAACTAAGAAGACAGCTTGTCCACAACAAAATAAAGAGAAACCGATTTTGGAAATCAGTGGTTAACCCCAAAACCCCTTCTACTTCGTTCTCACTTACCCGATGGTGAGGCATCCCGACTGCGCTGCATCTCCGCGGAATTGGCCTTGCTGGAAGCGGAGGCACTACTGCCCGAACTGATGT

 

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