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CRIMIC CR70269

Gene CG17739

CG17739

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:12306528..12310412	48F6-48F7	FBti0217696

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCACCGATAGTGGTCCATCATA	GCGTTGACAGCTTATGGCATCTTG

Images

Construct Design Sequences

sgRNA sequence	TATATACTTTAATCCTGTAGCGG
sgRNA2 sequence	AACTATGTAATCATTACCAATGG

 

left_200bp	right_200bp
GTGCGCCGCATCAAATCAAATTTCCCCAACGATCCCCGTTCGCCCTTTTATGATCCCACTGGTGCCCAGATGAAGCCATTGGCCACCCTGCACATCAATCGAAGACGACTCTACGAGAAGAACTGCGAGTCCTCCGATTGTAAGTTAAATGCAACCATATATTGATGTTGTGAATATACATACTATATACTTTAATCCTG	CAATGGATATCGTTAAATATATGAAAAATTCCAGACTACCTACACTAGCTCTTCTCGCAACAGATTAAATATGTATTAAAAACAATAAAACAGAATTGTGTTCGCGTGGTAATTCCAATTCCGATTGAGTTTAACCTTGACCCAGTTCCATTAAGCAAACAGTGGCCAGTTTACGAGTGATTTTTCGCCGGGTTGCAAGT

 

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