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CRIMIC CR70270

Gene GLS

CG42708

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	2R:12670483..12679593		FBti0220442

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCAATTCCATGATTCCCAGTGTAG	ACATACGTGTAGAAGGGGAACGAG

Images

Construct Design Sequences

sgRNA sequence	ACGATGGTCTCTCTGATTCACGG
sgRNA2 sequence	GAAGGATCACGACTTGCACGTGG

 

left_200bp	right_200bp
CTGCTTTTGCCGCCGCCAACGTTTTTTGCGGCAGCGTTGAGCTGATTATGAGCTTTCTGCTTTTGGCATTTTAGTACCCAGCGATTGGTAAAAGAGGACGCATGTGCAGACAACGGAGCGGATACGCAGCGTGAACGTGAACGAACACATCAAGTACGAGCATTAAGGTTTACCCCTGTTAGGCATCCGATAATCCGTGA	GCAAGTCGTGATCCTTCGAGGCGGATCCACTGCACGAGCGGCATTTGTACTTGTACTTGGTAGCGCTTAGTTAGCCACAAAAATTCCATGTTATCCTACGAAACAATGACAATAATCTTAACGGATATCTAACTTTCCCACACAACTGGGGTCTGTGACCTGCCTAAATGTATTGCATTCGTACTTGTCAATTGATATTT

 

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