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CRIMIC CR70273

Gene Arc2

CG13941

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	2R:14361924..14362825	50F6	FBti0220445

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	OK	TCCTGGTTGTTGTGCTCGATGATG	CCTGCAACCTGAAAGAGCATGA

Images

Construct Design Sequences

sgRNA sequence	CCCCCCAACCGTCTAACGATTGG
sgRNA2 sequence	TTAGTGCAATCTGTGTTTATTGG

 

left_200bp	right_200bp
CACGTGGTGGCCTCCTTGCGGAGGAACCCCCACAAACTGTGCTCCCCAATTATTCCGAAATGTTGAAGAGAGAGAGAGTGTTGGATATTTTTCGAAACTCGAAAAAGCTTTTACTTACAGAGAAAGAGAGAGGGAGGAGAGCAATGTGGCTTTTTATTTCACAACAAGCGAAATCGCCAGTTGCCCCCCAACCGTCTAAC	AACACAGATTGCACTAAATAAATCTCTTAAGCGCCTTTATTGGGCAGATTTTAAATAACTTAATAACTACATGTATAAACCATTTATTTTTTCATTTATAAGCTTTAAGATATGATTCATTCAATGATAAGTATCAGCATATACAAATAAATAAATATACGATGATATATGATAATAAGAAGAAACTGACTGAAAATAGG

 

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