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CRIMIC CR70277

Gene Arc1

CG12505

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	2R:14357929..14360297	50F6-50F6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CGGTGAATGCCCACTTAGATCCTT	CGTTTTCGAGGCATTGCGAATAGC

Images

Construct Design Sequences

sgRNA sequence	TTAGGTGATCAAATTAATCTTGG
sgRNA2 sequence	AAAGAACATCAAATACCAACAGG

 

left_200bp	right_200bp
TTCGCGATGATTCACTGTGAGCAATAATACCAAAAACATAGACCATCTCTCCCTTATTGGCTCTCCCCAGTGCCTCAAGCTTTTTCGAAGTTCGATTTCACAACAGGCGGATATAAAAGGGCTGCCATGTGGGAGAGCTGTTCAGTTCAAATCACCGGCCGCATTCGCTACACTGGCTTTGTCCGCCGACTGAACCAAGA	AACAGGCAGCAGCCAACGTCATTGATGACCTCATCGCTGCTGCCGCCCACCCAAACAGAACTTTTCGTAGCCTCGCCAGCCGATCGACGTCAGAGAGAGCCCAACAACCGGAGAGCTGGAGATGGGGAGCAGCCACATCAACAGCAGCAACAGCAGCCACATCTCTTCATACTCTTCATACTCACTGGCAATCGGCGTGC

 

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