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CRIMIC CR70279

Gene whip

CG34218

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_kG4	2R:8362359..8363218		FBti0220449	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTTGCGGAAAGAATGAGACGCGA	GGTACTCACGCAACATGAGTATCG

Images

Construct Design Sequences

sgRNA sequence	CAGAAGAAGCTAGCGAGAACTGG
sgRNA2 sequence	TAAAATGAGCGTGGAGTAATTGG

 

left_200bp	right_200bp
TTGGATTTTTAGTATTTTACATCAAGGTCACCCACGCCATTGCAGTCATCTATAAAATGAATCGATTATTTCGGAGTCACCCATTTTTTAATCAAGCAACAGGCTTCCAAATCGAACGTTTCCATTTAGATACTACCCAACCCATCTCTCACGCAGACCGAAGCATTCAGGGAAACTAAGCAGCAGAAGAAGCTAGCGAG	ACTCCACGCTCATTTTAGCGTAGTGATTGGGTTGCACTTGCGATTGGCATCAGGGGTCCAAGATTCTCCACAATTAGCCGTATTAAAGATGTAATGTGTAAAGCAATAGTCTTTTTAATCATTGGCCTATATACGGAGTGAAGTCTGGTAATGAATACTGTAGAATGTATTCAGCAGCTTAATTTTGTAGACCAAACTTG

 

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