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CRIMIC CR70281

Gene CrebA

CG7450

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:11535395..15553214	71E1	FBti0217680

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACTTGTTCTTGCTGTTGTACACGG	GCGATCTTGATCTTACTTCCCAGACC

Images

Construct Design Sequences

sgRNA sequence	TCTACAAGTCGAGTAGTCGCCGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAAAGAATCTATTACAGTTTGGGAGGAGGACACATGTGGAGTTGATAAGAGGCTGCTATCAGCTGGTCTAAATGCAAAGTAATGACTTTTTTCGCCTGCCTGCCTGCCTGCTGCTGCTGCTGCTGCACTTTGCAGCTAGTCAAATAGAGTATGGCTCGTCAGTGTCATTACTATTGGGAGATAATAAATGCGCGCCGGCG	ACTACTCGACTTGTAGACAATGTAGCCGGAGGGACAGAAATAGGCCCAATGCCCGGGAAAATGCGCGTGCAGTTTTTATTGGTTTTGTTGCATTGCGTTTTTTCCCGCCGTAAGCGGTACAATGTACCCGTACCCGGGGCGTGCGCTCAATTACCTCATAAGTATCTCAAATGCCTGTGTGTGCCCATAACAAGAAATTA

 

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