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CRIMIC CR70287

Gene kis

CG3696

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:210735..250823	21B4-21B5	FBti0217683

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGTAAGGCAGGTCCACACTAAAC	GGTGTATCCTTGTGATTCCATGCC

Images

Construct Design Sequences

sgRNA sequence	CTGGCTATGAACATGGCTACAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGTGGTTTCAAGTATTATTATTATTTAATTGATCTACTCTTAATCAATTTCTTAAAAACCACTTTGTGCAACGGTTATATCTATTTATTGCCTTTTAAGTGCACTCACTCAAATTCTTTTTTGCGCGGCTGTTTATTGCTCCGCGTTCTCTATTTTATTTACCCTATTGCTATTTACCTACCGCCGCCTCCAAGCCTGTA	GCCATGTTCATAGCCAGCGGTTCTACTAATATTCATAATCAATCGCTCAAGTCGCCTCGCACTTGTGTAGTGTAAATGCGATGGTGGAACGGTGCGGAGAAAACGAGTGATAGAGAGGCGGAGTGAGTGCGAGCGAGAGCCAAATGTATAGTACGCGAGAAGGGAAGAAAGAAGAGGCCTCGAGTCGCTGTCGTGTTATC

 

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