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CRIMIC CR70288

Gene Apc2

CG6193

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:24163966..24168264	95E6	FBti0220980

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GCAGAGTTGTCAACAGTTGTCGGT	TACCGAATGCCAAGACATTCGAGC

Images

Construct Design Sequences

sgRNA sequence	ATTCGGATACCAGGGATTCTGGG
sgRNA2 sequence	GCGTCAGCACTAGAAGCGGATGG

 

left_200bp	right_200bp
ATCATTGAACTTGGGCAGAACTAGCTATAAATTAGCTTAATCGGTAGCACTGGAGCGCTGAGCCTGCGCGCTTGGAAAATGGCTTAAACGCAGGCCATCTCAAACAAAGATCGAGGATCTCAGATCAGAAGGAACTACCTGTTGAAGCGACCGTGCAACAGGACTCATCCAGAAGCAAAGCTGCAAAGGTTTATTCCCAGA	GGATGGTTGGTTAATTGAGAAGCGCATGCATTTGTTTAGTGGCTTTTAATCTTGTCTACGCAATAATATTCTCACGGTCTCAATTTTCCTATAGTTCAGATTAAAAATTTGCACTTAAATTTAACTCTCCAACACGTGTAATATTATGTTTACCCTCTCACTAAACAATTAAGGAAACTAAAAAGGGTGATAAGAAATGG

 

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