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CRIMIC CR70289

Gene erm

CG31670

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:1950239..1970362	22B6-22B7	FBti0217684

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATAAGCTCGATGTGGTTCGCACTC	AAACGCAAACCGCAGTGCGTTCCATA

Images

Construct Design Sequences

sgRNA sequence	CTGCGGACCCAGATGGGAGCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAACATAATTCGGCTGCTTAAAAGTGGAACTACTCGTTCGTAGTAGTAGTAGTTTGGCACCCGGGAAACTTTGGGATCGAGGGCTGACCGCCGCTGCCTCCGGGTCAGCGTGTGAAATGGTTGCCGCCTCTCGTTTAATGAGATTAAAGCGCACTTTGGAAGGGAAACTGCAAGATGGGCGAACTGCGGACCCAGATGGG	AGCTGGGCCTGCTGAGGCGTCAAATCAAGTTAATTAAAAGCGGCAAGGAAGTCGGTAATCGCACCGGATGTCGCTCATTAGCCACACTCGAGGGCACATGGGGAAGATGGAGCCGGCGAGGCGGCGAGGCGGCCTTACACCTCCAGTCGAGTGTAAGCGATTTAATGAATCTCACATAAAGCCAAGACCCGCGCCAAAAA

 

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