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CRIMIC CR70292

Gene Seipin

CG9904

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	X:2619799..2622140	3A7-3A8	FBti0220951

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCTGGGCTGGAATGTCTACGATC	ATCGAGTCGTAGTCCCTCATCT

Images

Construct Design Sequences

sgRNA sequence	ATGTACATTTATCTAAATATAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCGGCTGGGCCTCATCGCCTTCGCCGTGGTGCTCATCATCTGGCTGGCGGTgTTCATGTACGCCGCCTTCTACTACGTCTACATGCCGGCCATATCGCACACCCGACCCGTTCACATGCAGTTCAAGTGAGTAGCTTCGAATGTGAAGGAAATCGATGTGGCTACAGGTTTTCTTCCTGTGCCATGTACATTTATCTAAA	TATAGGCCATTTGTCCATAAATATATGTAGACCTAATCTGCTTATCAGTCCATGCGATTGGCTATTGCAAGCTAGTTGGTAATTGTCAAGTTGTTTGATTCATAGTTAGCCGCTCTAGACTTAGTGCCCAATAAAACAATGCCGTTGAATTCCGCAGAACCTGCCTGGAGACCAGCACGCCCTGCACCTTTCCGCACGCG

 

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