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CRIMIC CR70296

Gene Yif1

CG5484

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:26717871..26719806	9C75	FBti0220952	95026

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCAATAAGATGAACAATAGGAAA	TAACATCACTCACACGCTTCGG

Images

Construct Design Sequences

sgRNA sequence	AAATTCCGGAGTGGCTGCACCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CATGCTTTGGTATTTGGCAACACTAAACTCCGGTCACACAACAAAATGCTTATGGTGATGTGTTAAAAAGCGAAATTTTGAATACAATTTACTGATCCAGGAAGATGAACTACAATCCAAATCCGGGTATGCGGAACCGTAAGTGGGAGAACTGTAAGGATCAGGCTACGCAAGCAAGTATACCCGAAGGATGTCCGGTG	CAGCCACTCCGGAATTTCCCCACCCGCCGCTGACCGGAATCGGAATCCGGAGTGCTGTCACTGTTTACGTTACACGTTACCCCGTTTTGTGCGCCGATCCTGGCTTCCACTGCATCCTGCCCCCCAGTTTCTATGCCTGCCCTGCGTTTCCGTTCCGTGCTGCGTGTGTAGCTTGAGTGCCTGTGTTCCCCGCTTCCCAG

 

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