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CRIMIC CR70297

Gene Tom70

CG6756

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:12035984..12038511	33B9-33B10	FBti0220983	95027

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTCGATGTCGTGTGCTCAGCA	ACTACAAGCTGGTGAGCAGTGTGA

Images

Construct Design Sequences

sgRNA sequence	GAAAAGAGCTGAATCAGTTTGGG
sgRNA2 sequence	CTGGGCCATCAATCCGGATTGGG

 

left_200bp	right_200bp
CAGTGTAAAGTTTTAAAAAAGTGTAAGTTCCGTTCGCTGACTGCACTGATTCACATCCCGGCCAGTTGGGGGTGTGAAAAATCAATTGGGTTTGGGGTTAGGTACTGCAGAAACGTGCCCCCCGCCCGGCATTTAGCAAGCTCAAAAGGCGCTGAGCGATAAGCCAGCAGAGTATTACACAATTTGTGGCGGCACCCAAA	TGTTGGGATTGATATCCCGAGAAAATAAGAGCCAGCGCGGAGCATTTAGCCAGCAGTCCTACTCTAATTAAGTTAATATCGACCATACCTGTTATTTATATATATATATATAAAGCTTGTTTACGTTTATGCTCCTAGCTTTAGCATAGAAATTGTCATGTATTATGGTACATATGTAAACTAGAATCTGTTGCCTAAGG

 

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