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CRIMIC CR70298

Gene RhoGAP100F

CG1976

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:31812609..31846570	100D2-100D3	FBti0217685

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCACATAGTCACCCGAATCCCAAT	GACTTGCTTAACGGAAACAGGC

Images

Construct Design Sequences

sgRNA sequence	TGTTGGCTGAACGTCAATTAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCACCGCGCACGCAGTGAAATTGTCTTTTGTCTATGAATATAAATATGCTCCTGTATGTGTCACACATTTACCCACTTGAAAACAAACATTAACATTAACACTAAGCCCCGAAATGATTCAAAATTTGAAATTCTTACTGCACCACCCAAAACCAAGAATTTGCACGTATTGACGCCCTTAAAGCTACTTTCAGCCTTAA	TTGACGTTCAGCCAACATATAACCAACTTTGCGGGAATTCCCCTGCTCTCCTTTCCGTTCGTTATTTTCTTGCAGCCCTATCAATTGGGGGGCTCAGTGGGCTCAGCAATTCCCGACCCATCGCCACTGCCACTTCCAGGCACACCCTCCCCCGGCAGTAGCTCAGCATCGACGGGCTCAGGCTCCGGATCGGGTAAAAG

 

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